Sequencing 2’-O-methylation RiboMeth-seq, Alkaline hydrolysis (illumina, nanopore) RibOxi-seq, (Periodate oxidation Nm-seq, Periodate oxidation 2OMe-seq, RT at low dNTP MeTH-seq, RT at low Mg2+ Nm-Mut-seq, Engineered RTase NJU-seq, MgR cleavage Relative

MGI Nanopore CycloneSEQ-WT02, 4,096 nanopore proteins per flow cell, with a maximum single-flow cell throughput of 50 Gb and up to 100 Gb for the dual flow cell system CycloneSEQ-WY01, over 30,000 nanopores proteins per flow cell News

Several new RT enzymes TGIRT INDURO ultraMarathon BomoC ottr-seq

RNA Cap structure Cap0 Cap1 Cap2 ##Co-transcription ##Cleancap protocol Cap 1 Messenger RNA Synthesis with Co transcriptional CleanCap® Analog by In Vitro Transcription ##CleanCap ref1 ref2 ref3

Single cell methods Plate based FCAS LCM Fluidigm C1 Microfluidics Fluidigm C1 nanowell (microwellseq, Seqwell, Icell8, Biorad…) Droplet based (indrop, dropseq, 10x chromium…) Split-pool Parse-seq Split-seq Device free pip-seq Revseq ref1