Methods to study multiple targets simutaneously #TFs Simultaneous profiling of multiple chromatin proteins in the same cells Multifactorial chromatin regulatory landscapes at single cell resolution Nanobody-tethered transposition allows for multifactorial chromatin profiling at single-cell resolution NTT-Seq kit #RBPs CLIP-IT: A massively...

GalNAc From the pipeline table, it is very clear that the siRNA therapeutics are using more specific GalNac for delivering to the liver. Dr. Muthiah Mano developed GalNac and received the lifetime achievement award at Oligonucleotide Therapeutics Society (OTS) in 2019. This chemical modification binds to the Asialoglycoprotein receptor that is...

List of nucleic acid drugs in clinical trials Reference Nucleic acid Name Company Nanoparticle system/ Disease Clinical trial ID number ASO Pelacarsen (AKCEA-APO(a)-LRx) Ionis, Akcea, and Novartis Gen 2+ (LICA) Hyperlipoproteinemia(a) and cardiovasc...

lipids in patisiran The ionizable cationic lipid such as DODAP has pKa < 7 and can efficiently encapsulate negatively charged nucleic acids at low pH (pH 4) during the ethanol loading procedure. On mixing the nucleic acids with lipid in ethanol, they form inverted micelle containing nucleic acids surrounded by cationic lipid. If the mixing ...

lipids in mRNA vaccine The mRNA vaccine contains 2 core components, the Lipid Nanoparticle (LNP) wraps the core mRNA (~4.18kb). Upon intramuscular injection, the mRNAs were taken by the immune cells and translate into covid-19 (NC_045512.2) spike protein. Both Pfizer and Moderna encodes 100% identical full length (NC_045512.2) spike protein, b...